Gene Name Synonym:
CD340; ENV; ENVW; ERVWE1; HER-2; HER-2/neu; HER2; HERV-7q;HERV-W-ENV; HERV7Q; HERVW; HERVWENV; MLN 19; MLN19; NEU;NGL; TKR1 純度：> 90 % as determined by SDS-PAGE
內(nèi)毒素：< 1.0 EU per μg of the protein as determined by the LAL method
表達(dá)宿主：HEK293 Cells
種屬：Human
預(yù)測 ：N 端Thr 23
分子量：The recombinant human ErbB2 comprises 641 amino acids and has a calculated molecular mass of 71 kDa. As a result of glycosylation, rh ErbB2 migrates as an approximately 100-110 kDa protein in SDS-PAGE under reducing conditions.
生物活性
1. Measured by its binding ability in a functional ELISA.
Immobilized human Erbb2 at 2.5 μg/ml (100 μl/well) can bind Herceptin with a linear range of 1.28-32 ng/ml.
2. Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.2-1 μg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.
3. Using the Octet RED System, the affinity constant (Kd) of Her2 / ERBB2 Protein, Human, Recombinant (His Tag) (Cat: 10004-H08H) bound to Pertuzumab was 0.1 nM.
蛋白構(gòu)建：
A DNA sequence encoding the extracellular domain (Met 1-Thr 652) of human ErbB2 (NP_004439.2) was fused with the polyhistidine tag at the C-terminus.
緩沖液：
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
運輸方式：
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
穩(wěn)定性 & 儲存條件：
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
復(fù)溶：
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.